| 論文名 |
Preparation of branched cyclomaltoheptaose with 3-O-α-L-fucopyranosyl-α-D-mannopyranose and changes in fucosylation of HCT116 cells treated with the fucose-modified cyclomaltoheptaose. |
| 著者 |
Madoka Kimura
Yuki Masui
Yuko Shirai
Chie Honda
Kenta Moriwaki
Taku Imai
Uichiro Takagi
Takaaki Kiryu
Taro Kiso
Hiromi Murakami
Hirofumi Nakano
Sumio Kitahata
Eiji Miyoshi
Toshiko Tanimoto
|
| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Carbohydr Res. 2013 Jun 7;374:49-58. |
| WEBサイト |
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| 論文概要（和文） |
3-O-α-L-フコピラノシル-α-D-マンノピラノースを用いた分岐シクロマルトヘプタオースの調製と、フコース修飾シクロマルトヘプタオースで処理したHCT116細胞のフコシル化の変化。 |
| 論文概要（英文） |
From a mixture of 4-nitrophenyl α-L-fucopyranoside and D-mannopyranose, 3-O-α-L-fucopyranosyl-D-mannopyranose was synthesised through the transferring action of α-fucosidase (Sumizyme PHY). 6(I),6(IV)-Di-O-(3-O-α-L-fucopyranosyl-α-D-mannopyranosyl)-cyclomaltoheptaose {8, 6(I),6(IV)-di-O-[α-L-Fuc-(1→3)-α-D-Man]-βCD} was chemically synthesised using the trichloroacetimidate method. The structures were confirmed by MS and NMR spectroscopy. A cell-based assay using the fucosyl βCD derivatives, including the newly synthesised 8, showed that derivatives with two branches of the α-L-Fuc or α-L-Fuc-(1→3)-α-D-Man residues possessed slight growth-promoting effects and lower toxicity in HCT116 cells compared to those with one branch. These compounds may be useful as drug carriers in targeted drug delivery systems. |
