シクロデキストリン論文データベース検索システム
| 論文名 | Preparation of soluble stable C??/human serum albumin nanoparticles via cyclodextrin complexation and their reactive oxygen production characteristics. |
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| 著者 | Altaf Abdulmalik Aldawsari Hibah Banjar M Zainy Anraku Makoto Iohara Daisuke Otagiri Masaki Uekama Kaneto Hirayama Fumitoshi |
| キーワード | |
| 出版年月 | 1970年1月 |
| 発表先 | Life Sci. 2013 Aug 28;93(7):277-82. |
| WEBサイト | |
| 論文概要（和文） | シクロデキストリン錯体化による可溶性安定C??/ヒト血清アルブミンナノ粒子の調製およびそれらの反応性酸素生成特性。 |
| 論文概要（英文） | Aims: The stability of solutions of fullerene C?? with human serum albumin (C??/HSA) has not been studied in detail. In this study, we report on the preparation of stable C??/HSA solutions that are formed via the formation of C??/HP-β-CyD nanoparticles, i.e. by transferring C?? molecules from HP-β-CyD to HSA molecules, and an investigation of the reactive oxygen species on the behavior of the resulting C??/HSA. Main methods: Structural and functional properties were examined by spectroscopic techniques, including circular dichroism (CD), fluorescent spectra (FI), and electron spin resonance spin-trapping method, and by cell viability test using A549 cells. Key finding: Aqueous C??/HSA solutions with a small distribution size, excellent dispersion stability and a high dispersion concentration were obtained without the use of organic solvents. CD and FI spectra indicated that the HSA had undergone structural changes as the result of C??/HSA formation. Binding experiments indicated that C??/HSA had increased Site I and Site II-ligand binding capabilities. The HSA, in the form of C??/HSA was, therefore, conformationally altered, and its binding capabilities were enhanced. Radical scavenging studies using the 1,1'-diphenyl-2-picrylhydrazyl radical showed that C??/HSA had an increased antioxidant activity, compared to HSA alone. Further, C??/HSA efficiently generated not only superoxide anion radicals O??- but also singlet oxygen ?O? through photoirradiation. C??/HSA showed cell toxicity characteristics after light irradiation, but no toxicity was observed in the absence of irradiation. Significance: C??/HSA not only has an excellent stability and antioxidant activity, but also has substantial phototoxicity properties. It thus appears that HSA can be used as a carrier of C??in drug delivery systems for photodynamic therapy. |