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論文名	Purification and characterization of an amylase from Opuntiaficus-indica seeds.
著者	Monia Ennouri Bassem Khemakhem Hanen Ben Hassen Imen Ammar Karima Belghith Hamadi Attia
キーワード
出版年月	1970年1月
発表先	J Sci Food Agric. 2013 Jan 15;93(1):61-6.
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論文概要（和文）	Opuntiaficus-indica種子からのアミラーゼの精製と特性化。
論文概要（英文）	Background: In Tunisia, prickly pear fruit grow spontaneously; it is consumed as fresh fruit, juice or jam. When the fruit is used for juice production, the seeds are discarded and go to waste. Our study aimed to extract biomolecules from seeds by producing value-added products from the fruits. Results: An amylase from Opuntia ficus-indica seeds was extracted and purified to homogeneity. An increase in specific activity of 113-fold was observed. The apparent molecular mass of the enzyme is 64 kDa. The optimum pH and temperature for enzyme activity were pH 5 and 60 °C, respectively. Under these conditions, the specific activity is 245.5 U mg(-1) . The enzyme was activated by Co(2+) and Mg(2+) (relative activity 117% and 113% respectively) at lower ion concentrations. It was strongly inhibited by Mn(2+) and Fe(2+) . Cu(2+) inhibited totally the activity of this enzyme, but Ca(2+) has an inhibitory effect which increases with ion concentration. Conclusion: The extracted enzyme belongs to the exo type of amylases and is classified as a β-cyclodextrin glycosyltransferase since it generates mainly β-cyclodextrin from starch. It exhibits high thermal stability and a broad range of pH stability, making it a promising prospect for industrial and food applications.