| 論文名 |
Purification, crystallization and preliminary crystallographic analysis of the marine α-amylase AmyP. |
| 著者 |
Jigang Yu
Chengliang Wang
Yanjin Hu
Yuanqiu Dong
Ying Wang
Xiaoming Tu
Hui Peng
Xuecheng Zhang
|
| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):263-6. |
| WEBサイト |
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| 論文概要（和文） |
海洋α-アミラーゼAmyPの精製、結晶化および予備結晶解析。 |
| 論文概要（英文） |
AmyP is a raw-starch-degrading α-amylase newly identified from a marine metagenome library. It shares low sequence similarity with characterized glycoside hydrolases and was classified into a new subfamily of GH13. In particular, it showed preferential degradation to raw rice starch. Full-length AmyP was cloned and overexpressed in Escherichia coli, then purified and crystallized in the presence of its substrate analogue β-cyclodextrin. X-ray diffraction data were collected to a resolution of 2.1 ?. The crystal belonged to space group P2?2?2, with unit-cell parameters a=129.824, b=215.534, c=79.699 ?, α=β=γ=90°, and was estimated to contain two molecules in one asymmetric unit. |
