| 論文名 |
Aptamer-based single-molecule imaging of insulin receptors in living cells. |
| 著者 |
Minhyeok Chang
Mijin Kwon
Sooran Kim
Na-Oh Yunn
Daehyung Kim
Sung Ho Ryu
Jong-Bong Lee
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
J Biomed Opt. 2014 May;19(5):051204. doi: 10.1117/1.JBO.19.5.051204. |
| WEBサイト |
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| 論文概要（和文） |
生きている細胞におけるインスリン受容体のアプタマーベースの単一分子イメージング |
| 論文概要（英文） |
We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-β-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane. |
