| 論文名 |
A novel actin cytoskeleton-dependent noncaveolar microdomain composed of homo-oligomeric caveolin-2 for activation of insulin signaling. |
| 著者 |
Hayeong Kwon
Jaewoong Lee
Kyuho Jeong
Donghwan Jang
Yunbae Pak
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Biochim Biophys Acta. 2013 Oct;1833(10):2176-89. |
| WEBサイト |
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| 論文概要（和文） |
インスリンシグナル伝達の活性化のためのホモオリゴマーカベオリン-2から構成される新規アクチン細胞骨格依存性非カベオラマイクロドメイン。 |
| 論文概要（英文） |
The role of caveolin-2 (cav-2), independently of caveolin-1 (cav-1) and caveolae, has remained elusive. Our data show that cav-2 exists in the plasma membrane (PM) in cells lacking cav-1 and forms homo-oligomeric complexes. Cav-2 did not interact with cavin-1 and cavin-2 in the PM. Rab6-GTP was required for the microtubule-dependent exocytic transport of cav-2 from the Golgi to the PM independently of cav-1. The cav-2-oligomerized noncaveolar microdomain was unaffected by cholesterol depletion and protected from shearing of silica-coated PM. Activation of insulin receptor (IR) was processed in the microdomain. Actin depolymerization affected the formation and sustenance of cav-2-oligomerized noncaveolar microdomain and attenuated IR recruitment to the microdomain thereby inhibiting IR signaling activation. Cav-2 shRNA stable cells and the cells ectopically expressing an oligomerization domain truncation mutant, cav-2?47-86 exhibited retardation of IR signaling activation via the noncaveolar microdomain. Elevation in status of cav-2 expression rendered the noncaveolar activation of IR signaling in cav-1 down-regulated or/and cholesterol-depleted cells. Our findings reveal a novel homo-oligomeric cav-2 microdomain responsible for regulating activation of IR signaling in the PM. |
