| 論文名 |
Continuous production of β-cyclodextrin from starch by highly stable cyclodextrin glycosyltransferase immobilized on chitosan. |
| 著者 |
J?ssie da Natividade Sch?ffer
Manuela Poletto Klein
Rafael C Rodrigues
Plinho Francisco Hertz
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Carbohydr Polym. 2013 Nov 6;98(2):1311-6. |
| WEBサイト |
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| 論文概要（和文） |
キトサンに固定化された高安定シクロデキストリン糖転移酵素によるデンプンからのβ-シクロデキストリンの連続生産。 |
| 論文概要（英文） |
Cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on glutaraldehyde-activated chitosan spheres and used in a packed bed reactor to investigate the continuous production of β-cyclodextrin (β-CD). The optimum temperatures were 75 °C and 85 °C at pH 6.0, respectively for free and immobilized CGTase, and the optimum pH (5.0) was the same for both at 60 °C. In the reactor, the effects of flow rate and substrate concentration in the β-CD production were evaluated. The optimum substrate concentration was 4% (w/v), maximizing the β-CD production (1.32 g/L) in a flow rate of 3 mL/min. In addition, the biocatalyst had good operational stability at 60 °C, maintaining 61% of its initial activity after 100 cycles of batch and 100% after 100 h of continuous use. These results suggest the possibility of using this immobilized biocatalyst in continuous production of CDs. |
