| 論文名 |
Effect of β-cyclodextrin complexation on solubility and enzymatic hydrolysis rate of icariin. |
| 著者 |
Li Cui
Zhenhai Zhang
E Sun
Xiaobin Jia
Qian Qian
|
| キーワード |
|
| 出版年月 |
1970年1月 |
| 発表先 |
J Nat Sci Biol Med. 2013 Jan;4(1):201-6. |
| WEBサイト |
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| 論文概要（和文） |
イカリインの溶解度と酵素加水分解速度に対するβ-シクロデキストリン錯化の影響。 |
| 論文概要（英文） |
Objective: The aim of this work was to investigate the effect of β-cyclodextrin complexation on the solubility and hydrolysis rate of icariin. Material and methods: The inclusion complex of icariin at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of icariin complex in water at 37°C was 36 times greater than that of free icariin. Enzymatic hydrolysis conditions were tested for the bioconversion of icariin by mono-factor experimental design. Methods: The inclusion complex of icariin at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of icariin complex in water at 37°C was 36 times greater than that of free icariin. Enzymatic hydrolysis conditions were tested for the bioconversion of icariin by mono-factor experimental design. Results: The enzymatic hydrolysis experiment showed that icariin can be transformed into baohuoside I. The optimum conditions determined were as follows: pH 5.0, 50°C, the ratio of cellulase/substrate (0.6), the concentration of icariin 20 mg/ml, and reaction time 12 h. Under these enzymatic conditions, 98.2% transforming rate of baohuoside I from icariin in inclusion complexes was obtained. Conclusion: The aqueous solubility and enzymatic hydrolysis rate of icariin were improved owing to the inclusion complexation. |
