| 論文名 |
Enantiomeric separation of R,S-tolterodine and R,S-methoxytolterodine with negatively charged cyclodextrins by capillary electrophoresis. |
| 著者 |
Petr Lehnert
Adam P?ibylka
V?t?zslav Maier
Joanna Znaleziona
Juraj ?ev??k
Michal Dou?a
|
| キーワード |
|
| 出版年月 |
1970年1月 |
| 発表先 |
J Sep Sci. 2013 May;36(9-10):1561-7. |
| WEBサイト |
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| 論文概要（和文） |
キャピラリー電気泳動による負に帯電したシクロデキストリンによるR、S-トルテロジンとR、S-メトキシトルテロジンの鏡像異性分離。 |
| 論文概要（英文） |
The methods for separation of R,S-tolterodine and R,S-methoxytolterodine enantiomers using sulfated α-, β-CD and phosphated-γ-CD by CE in acidic BGE based on Tris/phosphate pH 2.5 buffer were developed. Sulfated α- and β-CD allow anodic detection while phosphated-γ-CD allows only cathodic detection of the separated enantiomers. The influence of chiral selector (CS)'s concentration as well as the influence of composition and concentration of BGE on resolutions were studied. Reversal migration order of tolterodine and methoxytolterodine enantiomers was observed, when sulfated-α- and sulfated-β-CD were used. The developed methods with all three studied CSs, were validated and compared. All proposed methods enable determination of 0.2% of S-tolterodine as an optical impurity in pills, however the method with phosphated-γ-CD provided lower detection limit, better repeatability of peak areas and migration times, and also lower consumption of CS. Developed method employing phosphated-γ-CD that was applied for the determination of optical purity of R-tolterodine in commercial pills. |
