| 論文名 |
Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection. |
| 著者 |
Kaiying Liu
Li Wang
|
| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
J Chromatogr A. 2013 Jun 21;1295:142-6. |
| WEBサイト |
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| 論文概要（和文） |
532 Nmレーザー誘起蛍光検出を用いたキャピラリーアレイ電気泳動によるアミノ酸のエナンチオ分離。 |
| 論文概要（英文） |
Capillary array electrophoresis (CAE) is a promising technique for multiple enantiomeric separations. Carboxytetramethylrhodamine succinimidyl ester (TAMRA SE), a rhodamine-core fluorescent probe, has rarely been applied as an original precolumn derivatization reagent for chiral amino acid (AA) analysis so far. For these purposes, high-throughput enantiomeric separations of 12 TAMRA SE-AAs by a home-made 532 nm CAE-LIF scanner are presented. The effect of cyclodextrins (CDs) and a variety of organic modifiers was quickly investigated. Baseline separations were achieved in 100 mM Tris-borate buffer (pH 10.0) containing 2 mM β-CD and 10 mM hexamethylenediamine (HDA). Multiple determination of the enantiomeric excess (ee) in non-racemic mixtures of alanine is successfully presented. |
