| 論文名 |
Epidermal stem cells manipulated by pDNA-VEGF165/CYD-PEI nanoparticles loaded gelatin/β-TCP matrix as a therapeutic agent and gene delivery vehicle for wound healing. |
| 著者 |
Li-Hua Peng
Wei Wei
Xiao-Tian Qi
Ying-Hui Shan
Fang-Jun Zhang
Xi Chen
Qian-Ying Zhu
Lian Yu
Wen-Quan Liang
Jian-Qing Gao
|
| キーワード |
|
| 出版年月 |
1970年1月 |
| 発表先 |
Mol Pharm. 2013 Aug 5;10(8):3090-102. |
| WEBサイト |
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| 論文概要（和文） |
pDNA-VEGF165 / CYD-PEIナノ粒子によって操作された表皮幹細胞は、創傷治癒のための治療薬および遺伝子送達媒体としてゼラチン/β-TCPマトリックスを搭載。 |
| 論文概要（英文） |
The success of gene therapy largely relies on a safe and effective gene delivery system. The objective of this study is to design a highly efficient system for the transfection of epidermal stem cells (ESCs) and investigate the transfected ESCs (TESCs) as a therapeutic agent and gene delivery reservoir for wound treatment. As a nonviral vector, β-cyclodextrin-linked polyethylenimines (CYD-PEI) was synthesized by linking β-cyclodextrin with polyethylenimines (600 Da). Gelatin scaffold incorporating β-tricalcium phosphate (β-TCP) was utilized as a substrate for the culture and transfection of ESCs. With the CYD-PEI/pDNA-VEGF165 polyplexes incorporated gelatin/β-TCP scaffold based 3D transfection system, prolonged VEGF expression with a higher level was obtained at day 7 in ESCs than those in two-dimensional plates. Topical application of the TESCs significantly accelerated the skin re-epithelization, dermal collagen synthesis, and hair follicle regeneration. It also exhibited a potential in scar inhibition by regulating the distribution of different types of collagen. In contrast to ESCs, an additive capacity in stimulating angiogenesis at the wound site was observed in the TESCs. The present study provides a basis for the TESCs as a promising therapeutic agent and gene delivery reservoir for wound therapy. |
