| 論文名 |
Highlighting the role of polymer length, carbohydrate size, and nucleic acid type in potency of glycopolycation agents for pDNA and siRNA delivery. |
| 著者 |
Xue L
Ingle NP
Reineke TM
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Biomacromolecules. 2013 Nov 11;14(11):3903-15. |
| WEBサイト |
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| 論文概要（和文） |
pDNAおよびsiRNAデリバリーのためのグリコポリカチオン剤の効力におけるポリマーの長さ、炭水化物サイズ、および核酸タイプの役割を強調します。 |
| 論文概要（英文） |
A high resolution interferometric technique was used to determine swelling behavior of weakly charged polyacrylamide hydrogels in the presence of oppositely charged surfactants and subsequent exposure to cyclodextrins. Hydrogels of copolymerized acrylamide and 2-acrylamido-2-methyl-1-propanesulfonic acid (0.22, 0.44, 0.88 mol%) crosslinked with bisacrylamide (3, 6, 12 mol%) were employed. The equilibrium swelling and swelling kinetics of the hydrogels were determined with 2nm resolution of the optical length and sampled at approximately 1 Hz. These properties were determined for the hydrogels exposed to cationic surfactants dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB) at concentrations from 10(-7) up to 2×10(-3)M. The distribution of surfactant within one AAM-co-AMPSA hydrogel equilibrated in CTAB/perylene solution was investigated by confocal laser scanning microscopy. Hydrogels equilibrated at selected surfactant concentrations were subsequently exposed to cyclodextrins (α-CD, β-CD, methyl-β-CD and γ-CD) forming inclusion complexes with the surfactants. The results show different types of behavior for the two surfactants used, arising from the difference in the length of surfactant hydrophobic tail. The changes in the surfactant induced swelling of the hydrogels are suggested to arise from the net effect of electrostatic screening of sulfonic acid-amide group interactions and surfactant micellization. Hydrogels with the largest charge density and the lowest crosslink density yielded the most pronounced changes in swelling properties on exposure to DTAB or CTAB. The hydrogels displayed swelling kinetics on stepwise changes in surfactant concentrations that depended on the surfactant concentration range. The high resolution monitoring of hydrogel swelling associated with supramolecular complex formation in three-component systems hydrogel-amphiphilic molecule-cyclodextrin provides more details on the swelling behavior than previously disclosed. |
