| 論文名 |
Identification of nucleolin as a lipid-raft-dependent β1-integrin-interacting protein in A375 cell migration. |
| 著者 |
Jiajia Bi
Ruifei Wang
Yue Zhang
Xiaoqing Han
Khamal Kwesi Ampah
Wenguang Liu
Xianlu Zeng
|
| キーワード |
|
| 出版年月 |
1970年1月 |
| 発表先 |
Mol Cells. 2013 Dec;36(6):507-17. |
| WEBサイト |
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| 論文概要（和文） |
A375細胞遊走における脂質ラフト依存性β1インテグリン相互作用タンパク質としてのヌクレオリンの同定。 |
| 論文概要（英文） |
Lipid rafts are related to cell surface receptor function. Integrin is a major surface receptor protein in cell adhesion and migration on the extracellular matrix (ECM). Here, we showed that lipid rafts played a critical role in human melanoma A375 cell spreading and migration on fibronectin; an important component of the ECM that interacts with β1 integrin. We found that the disruption of lipid rafts did not markedly inhibit the expression and activation of β1 integrin. By coimmunoprecipitation and mass spectrometry, we investigated the influence of lipid rafts on the β1 integrin complex and identified nucleolin as a potential lipid-raft-dependent β1-integrin-interacting protein. Upon confirmation of the interaction between β1 integrin and nucleolin, further studies revealed that nucleolin colocalized with β1 integrin in lipid rafts and raft disruption interrupted their association. In addition, knockdown of nucleolin markedly attenuated A375 cell spreading and migration on fibronectin. Taken together, we demonstrated that nucleolin is a critical lipid-raft-dependent β1-integrin-interacting protein in A375 cell spreading and migration on fibronectin. |
