| 論文名 |
Improvement of cyclodextrin glycosyltransferase gene expression in Escherichia coli by insertion of regulatory sequences involved in the promotion of RNA transcription. |
| 著者 |
Norhayati Ramli
Suraini Abd-Aziz
Noorjahan Banu Alitheen
Mohd Ali Hassan
Toshinari Maeda
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Mol Biotechnol. 2013 Jul;54(3):961-8. |
| WEBサイト |
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| 論文概要（和文） |
RNA転写の促進に関与する調節配列の挿入による大腸菌におけるシクロデキストリングリコシルトランスフェラーゼ遺伝子発現の改善。 |
| 論文概要（英文） |
Regulation of RNA transcription in controlling the expression of genes at promoter and terminator regions is crucial as the interaction of RNA polymerase occurred at both sites. Gene encoding cyclodextrin glycosyltransferase (CGTase) from Bacillus sp. NR5 UPM isolated in the previous study was used for further construction of pTZCGT-SS, pTZCGT-BS and pTZCGT-BT expression systems for enhancement of CGTase production. The putative promoter regions, -35 and -10 sequences were found in the upstream of the mature gene start codon. Whereas, long inverted repeats sequences which can form a stable stem and loop structure was found downstream of the open reading frame (ORF) of Bacillus sp. NR5 UPM CGTase. The construction of E. coli strain harbouring pTZCGT-BS showed increment of 3.2-fold in CGTase activity compared to the wild type producer. However, insertion of terminator downstream of CGTase gene in E. coli strain harbouring pTZCGT-BT only resulted in 4.42 % increment of CGTase production compared to E. coli strain containing pTZCGT-BS, perhaps due to low intrinsic termination efficiency. Thus, it is suggested that the insertion of the putative promoter regions upstream of the coding sequence for the construction of CGTase expression system will further enhance in the recombinant enzyme production. |
