| 論文名 |
Improvement of regio-specific production of myricetin-3-O-α-L-rhamnoside in engineered Escherichia coli. |
| 著者 |
Nguyen Huy Thuan
Ramesh Prasad Pandey
Ta Thi Thu Thuy
Je Won Park
Jae Kyung Sohng
|
| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Appl Biochem Biotechnol. 2013 Dec;171(8):1956-67. |
| WEBサイト |
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| 論文概要（和文） |
人工大腸菌におけるミリセチン-3-O-α-L-ラムノシドの位置特異的生産の改善。 |
| 論文概要（英文） |
Myricetin is an important flavonol whose medically important properties include activities as an antioxidant, anticarcinogen, and antimutagen. The solubility, stability, and other biological properties of the compounds can be enhanced by conjugating aglycon with sugar moieties. The type of sugar moiety also plays a significant role in the biological and physical properties of the natural product glycosides. Reconstructed Escherichia coli containing thymidine diphosphate-α-L-rhamnose sugar gene cassette and Arabidopsis-derived glycosyltransferase were used for rhamnosylation of myricetin. Myricetin (100 μM) was exogenously supplemented to induced cultures of engineered E. coli. The formation of target product-myricetin-3-O-α-L-rhamnoside-was confirmed by chromatographic and NMR analyses. The yield of product was improved by using various mutants and methylated cyclodextrin as a molecular carrier for myricetin in combination with E. coli M3G3. The maximal yield of product is 55.6 μM (3.31-fold higher than the control E. coli MG3) and shows 55.6 % bioconversion of substrate under optimized conditions. |
