| 論文名 |
Inclusion Complex of Zerumbone with Hydroxypropyl- β -Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio. |
| 著者 |
Nabilah Muhammad Nadzri
Ahmad Bustamam Abdul
Mohd Aspollah Sukari
Siddig Ibrahim Abdelwahab
Eltayeb E M Eid
Syam Mohan
Behnam Kamalidehghan
Theebaa Anasamy
Kuan Beng Ng
Suvitha Syam
Ismail Adam Arbab
Heshu Sulaiman Rahman
Hapipah Mohd Ali
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Evid Based Complement Alternat Med
. 2013;2013:810632. |
| WEBサイト |
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| 論文概要（和文） |
ゼルンボーンとヒドロキシプロピル-β-シクロデキストリンの包接複合体は、カスパーゼ8 / BID切断スイッチおよびBcl2 / Bax比の調節を介して肝臓肝細胞HepG2細胞にアポトーシスを誘導します。 |
| 論文概要（英文） |
Zerumbone (ZER) isolated from Zingiber zerumbet was previously encapsulated with hydroxypropyl- β -cyclodextrin (HP β CD) to enhance ZER's solubility in water, thus making it highly tolerable in the human body. The anticancer effects of this new ZER-HP β CD inclusion complex via apoptosis cell death were assessed in this study for the first time in liver hepatocellular cells, HepG2. Apoptosis was ascertained by morphological study, nuclear stain, and sub-G1 cell population accumulation with G2/M arrest. Further investigations showed the release of cytochrome c and loss of mitochondrial membrane potential, proving mitochondrial dysfunction upon the ZER-HP β CD treatment as well as modulating proapoptotic and anti-apototic Bcl-2 family members. A significant increase in caspase 3/7, caspase 9, and caspase 8 was detected with the depletion of BID cleaved by caspase 8. Collectively, these results prove that a highly soluble inclusion complex of ZER-HP β CD could be a promising anticancer agent for the treatment of hepatocellular carcinoma in humans. |
