| 論文名 |
Inhibitory effects of β-cyclodextrin-helenalin complexes on H-TERT gene expression in the T47D breast cancer cell line - results of real time quantitative PCR. |
| 著者 |
Samaneh Ghasemali
Kazem Nejati-Koshki
Elham Tafsiri
Mohamad Rahmati-Yamchi
Abolfazl Akbarzadeh
Effat Alizadeh
Mozhgan Abbasi
Amin Barkhordari
Majid Tozihi
Shirafkan Kordi
Nosratollah Zarghami
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Asian Pac J Cancer Prev. 2013;14(11):6949-53. |
| WEBサイト |
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| 論文概要（和文） |
T47D乳癌細胞株におけるH-TERT遺伝子発現に対するβ-シクロデキストリン-ヘレナリン複合体の抑制効果-リアルタイム定量PCRの結果。 |
| 論文概要（英文） |
Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as a method for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulation in β-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnica chamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility and bioavailability. β-Cyclodextrin (β-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units, linked through 1,4-glycosidic bonds. Materials and methods: To test our hypothesis, we prepared β-cyclodextrin- helenalin complexes to determine their inhibitory effects on telomerase gene expression by real-time polymerase chain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assay showed that not only β-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that β-cyclodextrin- helenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of β-cyclodextrin-helenalin complexes increased. Conclusions: β-Cyclodextrin-helenalin complexes exerted cytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalin uptake by cells. Therefore, β-cyclodextrin could be superior carrier for this kind of hydrophobic agent. |
