| 論文名 |
Interaction of antitumor flavonoids with dsDNA in the absence and presence of Cu(II). |
| 著者 |
Yassin M Temerk
Mohamed S Ibrahim
Mohamed Kotb
Wolfgang Schuhmann
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Anal Bioanal Chem. 2013 Apr;405(11):3839-46. |
| WEBサイト |
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| 論文概要（和文） |
Cu（II）が存在しない場合と存在する場合の抗腫瘍フラボノイドとdsDNAの相互作用。 |
| 論文概要（英文） |
The binding of antitumor flavonoids, namely 3-hydroxyflavone (3HF) and hesperidin (Hesp) with dsDNA was investigated in the absence and presence of Cu(II) using cyclic voltammetry and square wave voltammetry at the hanging mercury drop electrode. The reduction currents of 3HF, 3HF-Cu complex, and the 3HF-β-cyclodextrin inclusion complex decreased after intercalation into dsDNA. The intercalation of Hesp into dsDNA is weak. dsDNA is reduced at a potential of -1.48 V overlaying the reduction of Hesp. In contrast, in the presence of Cu(II), the interaction of Hesp with dsDNA leads to a much stronger intercalation. The binding constants of the flavonoid-Cu complex with dsDNA were evaluated and calibration graphs for the determination of dsDNA were obtained from the decrease in the peak current in the cyclic voltammograms of 3HF in the presence of dsDNA. The proposed method exhibited good recovery and reproducibility for indirect determination of dsDNA. |
