| 論文名 |
Membrane cholesterol modulates the hyaluronan-binding ability of CD44 in T lymphocytes and controls rolling under shear flow. |
| 著者 |
Toshiyuki Murai
Chikara Sato
Mari Sato
Hidetoshi Nishiyama
Mitsuo Suga
Kazuhiro Mio
Hiroto Kawashima
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| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
J Cell Sci. 2013 Aug 1;126(Pt 15):3284-94. |
| WEBサイト |
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| 論文概要（和文） |
膜コレステロールは、Tリンパ球におけるCD44のヒアルロン酸結合能を調節し、せん断流下でのローリングを制御する。 |
| 論文概要（英文） |
The adhesion of circulating lymphocytes to the surface of vascular endothelial cells is important for their recruitment from blood to secondary lymphoid organs and to inflammatory sites. CD44 is a key adhesion molecule for this interaction and its ligand-binding ability is tightly regulated. Here we show that the hyaluronan-binding ability of CD44 in T cells is upregulated by the depletion of membrane cholesterol with methyl-β-cyclodextrin (MβCD), which disintegrates lipid rafts, i.e. cholesterol- and sphingolipid-enriched membrane microdomains. Increasing concentrations of MβCD led to a dose-dependent decrease in cellular cholesterol content and to upregulation of hyaluronan binding. Additionally, a cholesterol-binding agent filipin also increased hyaluronan binding. Cholesterol depletion caused CD44 to be dispersed from cholesterol-enriched membrane microdomains. Cholesterol depletion also increased the number of cells undergoing rolling adhesion under physiological flow conditions. Our results suggest that the ligand-binding ability of CD44 is governed by its cholesterol-dependent allocation to membrane microdomains at the cell surface. These findings provide novel insight into the regulation of T cell adhesion under blood flow. |
