| 論文名 |
Membrane localization of Jun?n virus glycoproteins requires cholesterol and cholesterol rich membranes. |
| 著者 |
Sandra M Cordo
Ayel?n Valko
Guadalupe M Martinez
N?lida A Candurra
|
| キーワード |
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| 出版年月 |
1970年1月 |
| 発表先 |
Biochem Biophys Res Commun. 2013 Jan 18;430(3):912-7. |
| WEBサイト |
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| 論文概要（和文） |
フニンウイルスの糖タンパク質の膜局在にはコレステロールおよびコレステロールに富む膜が必要。 |
| 論文概要（英文） |
Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Jun?n virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-β-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association. Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins. |
