| 論文名 |
Molecule-binding dependent assembly of split aptamer and γ-cyclodextrin: a sensitive excimer signaling approach for aptamer biosensors. |
| 著者 |
Fen Jin
Yan Lian
Jishan Li
Jing Zheng
Yaping Hu
Jinhua Liu
Jin Huang
Ronghua Yang
|
| キーワード |
|
| 出版年月 |
1970年1月 |
| 発表先 |
Anal Chim Acta. 2013 Oct 17;799:44-50. |
| WEBサイト |
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| 論文概要（和文） |
スプリットアプタマーとγ-シクロデキストリンの分子結合依存アセンブリ：アプタマーバイオセンサーのための高感度エキシマシグナリングアプローチ。 |
| 論文概要（英文） |
A highly sensitive and selective fluorescence aptamer biosensors for the determination of adenosine triphosphate (ATP) was developed. Binding of a target with splitting aptamers labeled with pyrene molecules form stable pyrene dimer in the γ-cyclodextrin (γ-CD) cavity, yielding a strong excimer emission. We have found that inclusion of pyrene dimer in γ-cyclodextrin cavity not only exhibits additive increases in quantum yield and emission lifetime of the excimer, but also facilitates target-induced fusion of the splitting aptamers to form the aptamer/target complex. As proof-of-principle, the approach was applied to fluorescence detection of adenosine triphosphate. With an anti-ATP aptamer, the approach exhibits excimer fluorescence response toward ATP with a maximum signal-to-background ratio of 32.1 and remarkably low detection limit of 80 nM ATP in buffer solution. Moreover, due to the additive fluorescence lifetime of excimer induced by γ-cyclodextrin, time-resolved measurements could be conveniently used to detect as low as 0.5 μM ATP in blood serum quantitatively. |
