| 論文名 |
Nanopore-based identification of individual nucleotides for direct RNA sequencing. |
| 著者 |
Mariam Ayub
Steven W Hardwick
Ben F Luisi
Hagan Bayley
|
| キーワード |
|
| 出版年月 |
1970年1月 |
| 発表先 |
Nano Lett. 2013;13(12):6144-50. |
| WEBサイト |
|
| 論文概要（和文） |
直接RNAシーケンスのための個々のヌクレオチドのNanoporeベースの識別。 |
| 論文概要（英文） |
We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially cleaved ribonucleoside diphosphates to a nanopore. |
